Fortessa FILTERconfig - Lund Stem Cell Center

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By:____Teia___. LASER: Blue Alt #1. Wavelength:__488__nm. Power:__50_mW. Bifoga ett 0,45 µm filter till en 10 mL-spruta. BD Fortessa Flow Cytometer, BD Biosciences, for flow cytometry analyss of samples.

Bd fortessa filters

Table of lasers and filters available: Laser Filters BD LSRFortessaTM Filters Features The standard configuration of the BD LSRFortessa™ can be changed in order to detect fluorophores with emission peaks not covered by the standard filter set. In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter… LSR Fortessa . The LSR Fortessa offers 5 lasers and is able to detect up to 18 parameters (fluorochromes) at the same time! Technical Highlights Lasers 355 nm, 20 mW laser power 405 nm solid state diode, 50 mW laser power 488 nm solid state, 50 mW laser power 561 nm, 640 nm , 40 mW laser power Flow cell 180 x 430µm rectangular quartz flow cell Configuration of Fortessa Laser Detector Band Pass Filter Dichoric Filter Color name available on the instrument Additional Color Channel in LSRII Blue 488nm (BV570 filter change) Yes F 450/50 Blank Pac Blue/V450/BV421 DAPI/Sytox Blue/Fixable Violet Yes Red 633nm If you change filter(s) on particular channel(s) for your experiment, please make sure to switch back to default settings and put the spare filter(s) back to the storage place; When you are done with the experiment be sure to wash BD LSRII (or BD LSR-Fortessa) properly: “Run” at … LSR Fortessa - software parameters and fluorochromes Laser Collection Filter (bandpass) Diva Parameter Name Fluorochromes 488nm Blue 530/30 530/30 Blue FITC, Alexa 488, eGFP, CFSE, Dye Cycle Green 670/30 30 Blue PerCp 710/50 710/50 Blue PerCP-Cy5.5, PerCp-eFluor 710, PerCp provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays. Boundless Potential The BD LSRFortessa Analyzer 4 5 BD High Throughput Sampler (HTS) BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a.

530/30. FITC/BB5815/VioBright FITC/Alexa488. 695/40.

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Violet Laser - 405nm 50 mW. PMT Feb 17, 2021 The BD LSR Fortessa has four laser lines: violet at 405 nm, blue at For the configuration click the link: BD LSR Fortessa X20 Filters and Collection filter, Mirror, Fluorochromes.

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Proteins. Proliferation BD Fortessa Configuration. KCRB. Room 4161.

We > think we have found a solution to this problem, and are wondering if anyone > else has seen it. 2013-06-27 · With the industry's most advanced high throughput plate loader and hotel, the S1000 family of analyzers go head-to-head with BD FACS Canto, BD LSR II, and BD Fortessa at a fraction of the cost and size while offering a superior solution for conventional and high throughput flow cytometry applications. To help with pinpointing exact data, the analyzer has the capacity to zoom in, allowing you easily visualize spectra intersection points with laser lines, filter sets and other spectra. To zoom in, double click your mouse over the area that you wish to zoom, or roll the mouse wheel forward. Catalog Number BD LSRFortessa X-20 This product is no longer available on Biocompare. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists.
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All monoclonal antibodies were obtained from BD PharMingen. After washing, cells were analyzed using LSRII Fortessa™ cell analyzer (BD Biosciences, CA), BCreative, BCW, BCX, BCXGS, BCxx, BD, BD Diesel Performance, BD home, BD Crafthouse Fortessa, Craftmade, Craftmill, CraftMore, Craftparts Direct Store, Fledging Store, Fleece, Fleece Performance, Fleetguard Cummins Filtration, Price search results for find the best price for with our price comparison tool. Prices updates daily! Detekteringsblocket i analysatorn Bd tillströmning.
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Filter ( nm). Example Fluorophores. (AF= Alexa Fluor). UV D. 379/28. BUV 395. UV C. Purge bubbles from sheath filter if necessary.